Journal of Advances in Nanomaterials

Download PDF (862.8 KB) PP. 37 - 43 Pub. Date: September 17, 2018

DOI: 10.22606/jan.2018.33001

• Asmathunisha. N^*
  CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamil Nadu, India
• Anburaj. R
  CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamil Nadu, India
• Kathiresan. K
  CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamil Nadu, India

The present study investigated the biosynthesis of silver and gold nanoparticles by marine cyanobacteria derived from coastal mangrove sediment of southeast India. Marine cyanobacteria were able to produce both silver and gold nanoparticles, as confirmed by visual observation, UV-Vis spectroscopy. Appearance of brown colour is an indication of silver nanoparticles, while ruby colour indicates the synthesis of gold nanoparticles in the reaction mixture. Control without silver nitrate and tetra chloro auric acid did not exhibit any colour change. The nanoparticles synthesized were mostly cubical, ranging in size from 70 to 85 nm for both silver and gold nanoparticles as evident by scanning electron microscopy (SEM) and Atomic force microscopy (AFM). The X-ray diffraction pattern confirmed the presence of crystallized nature of nanoparticles. Zeta potential value (-49.10 mV) of the silver nanoparticles and (-64.32 mV) of the gold nanoparticles revealed good stability of the nanoparticles up to 2 months. This work highlighted the possibility of using mangrove derived cyanobacteria for synthesis of silver and gold nanoparticles.

Silver, gold nanoparticles, mangroves, cyanobacteria, synechococcus elongatus

[1] Absar A, Satyajyoti S, Khan MI, Rajiv K, Sastry M (2005) Extra-/intracellular biosynthesis of gold nanoparticles by an alkalotolerant fungus, Trichothecium sp.J. Biomedical Nanotechnnology 1: 47-53.

[2] Ahmad A, Mukherjee P, Senapati S, Mandal D, Khan MI, Kumar R, Sastry M (2003) Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium Oxysporum. Colloids Surfaces B: Biointerfaces 27: 313-318.

[3] Asmathunisha N, Kathiresan K, Anburaj R, Nabeel M A (2010) Synthesis of antimicrobial silver nanoparticles by callus and leaf extracts from saltmarsh plant, Sesuvium portulacastrum L..Colloids Surf B Biointerfaces 79(2):488.

[4] Asmathunisha N (2010) Studies on synthesis of silver nanoparticles by mangroves, salt marshes and associated plants. Dissertation, Annamalai University, pp 98.

[5] Begum AN, Mondal S, Basu S, Laskar AR, Mandal D (2009) Biogenic synthesis of Au and Ag nanoparticlesusing aqueous solutions of Black Tea leaf extracts. Colloids Surfaces B. Biointerfaces 71: 113-118.

[6] Kathiresan K Manivannan S Nabeel M A Dhivya B (2009). Studies on silver nanoparticles synthesized by amarine fungus Penicillum fellutanum isolated from coastal mangrove sediment Colloids Surfaces B: Biointerfaces71: 133-137.

[7] Kathiresan K, Nabeel MA,Srimahibala P, Asmathunisha N, Saravanakumar K (2010) Analysis of antimicrobialsilver nanoparticles synthesized by coastal strains of Escherichia coli and Aspergillus niger. Can J Microbiol56(12):1050-1059.

[8] Lengke M, Fleet ME, Southam G (2006) Morphology of gold nanoparticles synthesized by filamentouscyanobacteria from gold (I)-thiosulfate and gold (III) - chloride complexes. Langmuir 22: 2780- 2787.

[9] Lengke M., Fleet, ME, Southam G (2007) Biosynthesis of silver nanoparticles by filamentous cyanobacteria afrom a silver(I) nitrate complex. Langmuir 23: 2694-2699.

[10] Monica F, Ioan IA, Constantin C, Simion A (2011) Interplay between gold nanoparticle biosynthesis andmetabolic activity of cyanobacterium Synechocystis sp. PCC 6803. Nanotechnology 22:485101.

[11] Mubarak Ali D, Sasikala M., Gunasekaran M,Thajuddin N (2010) Biosynthesis and characterization of Silvernanoparticles using marine cyanobacterium Oscillatoria willei NTDM01. Digest Journal of Nanomaterials andBiostructures 2: 415 - 420.