Isaac Scientific Publishing

Advances in Food Science and Engineering

Interferences of Sugarcane Glycoproteins on the Formation of Commercial Sucrose Crystals

Download PDF (683.3 KB) PP. 164 - 174 Pub. Date: December 13, 2017

DOI: 10.22606/afse.2017.14004

Author(s)

  • Elena Sánchez-Elordi
    Team of Cell Interactions in Plant Symbioses. Faculty of Biology, Complutense University. 12, José Antonio Novais Av., 28040 Madrid, (Spain)
  • María Blanch
    Department of Characterization, Quality and Security, Institute of Food Science Technology and Nutrition (ICTAN-CSIC), Madrid, Spain.
  • Carlos Vicente*
    Department of Characterization, Quality and Security, Institute of Food Science Technology and Nutrition (ICTAN-CSIC), Madrid, Spain.
  • María-Estrella Legaz
    Team of Cell Interactions in Plant Symbioses. Faculty of Biology, Complutense University. 12, José Antonio Novais Av., 28040 Madrid, (Spain)

Abstract

The aim of the present work is to study the way in which defense glycoproteins, produced by sugar cane plants, retard and modify the crystallization pattern of sucrose. The effect of defense glycoproteins against smut on the crystallization of sucrose has been studied with preference. In general, these glycoproteins delay the appearance of the first nuclei, hinder the association of individual crystals to form agglomerates or star-like nuclei, and increase the degree of surface erosion of the formed crystals. Using defense glycoproteins labeled with fluorescein isothiocyanate, it has been observed that the adhesion of these glycoproteins to the sucrose crystals is carried out mainly on the edges thereof, never on their flat faces, and never penetrates inside them, although they may move along its outer surface. The fluorescence diffuses in the zones of rupture or abrasion, indicating that the appearance of such accidents releases the proteins adhered to the free solution.

Keywords

Crystallization, glycoproteins, impurities, sucrose, sugar cane.

References

[1] J. Wang, S. Nayak, K. Koch, and R. Ming, “Carbon partitioning in sugarcane (Saccharum species),” Frontiers in Plant Science, vol. 4, paper 201, doi: 10.3389 / fpls.2013.00201, 2013.

[2] A. Whittaker, and F.C. Botha, “Carbon partitioning during sucrose Accumulation in sugarcane internodal tissue,” Plant Physiology, vol. 115, pp. 1651-1659, 1997.

[3] M.T. Covacevich, and G.N. Richards, “Studies on dextrans isolated from raw sugar manufactured from deteriorated cane. Part I. Isolation, purification and structure of dextrans,” International Sugar Journal, vol. 79, pp. 3-9, 1977.

[4] M.T. Covacevich, and Richards, “Studies on dextrans isolated from raw sugar manufactured from deteriorated cane. Part II. Determination of structure using a bacterial dextranase,” International Sugar Journal, vol. 79, pp. 33-37, 1977.

[5] E.J. Roberts, M.E. Godshall, F.G. Carpenter, and M.A. Clarke, “Composition of soluble indigenous polysaccharides from sugar cane,” International Sugar Journal, vol. 78, pp. 10-12, 1976.

[6] F.K. Imrice, and R.H. Tilbury, “Polysaccharides in sugar cane and its products,” Sugar Technology Reviews, vol. 1, pp. 291-361, 1972.

[7] P. Valdes, and C.W. Rodriguez, “Formación de polisacáridos en tallos de ca?a de azúcar recién cortados,” Ciencias de la Agricultura, vol. 12, pp. 45-52, 1982.

[8] P. Valdes, and C.W. Rodriguez, “Respuestas de los tallos de la ca?a de azúcar a los cortes,” Ciencias de la Agricultura, vol. 12, pp. 118-122, 1982.

[9] K. Schlumbach, A. Pautov, L. G?ckeritz, A. Bagherzadeh, and E. Fl?te, “Controlled sucrose crystallization at pilot-plant scale,” Sugar Industry, vol. 140, pp. 500–507, 2015.

[10] R. Broadfoot, and R.J. Steindl, “Solubility-crystallisation characteristics of Queensland molasses,” Proceedings of the International Society of Sugar Cane Technologists, vol. 17, pp. 2557-2581, 1980.

[11] P.W. Rein, and G.S. Cox, “Syrup clarification in raw sugar mills,” Proceedings of the South African Sugar Technology Association, vol. 61, pp. 22-31, 1987.

[12] E.J. Roberts, M.A. Clarke, M.E. Godshall, and F.W. Parris, “A glucan from sugar cane,” International Sugar Journal, vol. 87, pp. 227-231, 1985.

[13] D.B.S. Chauhan, O.P. Gupta, S. Kumar, and V. Kumar, “Identification of polysaccharides from Indian sugar,” Journal of Applicable Chemistry, vol. 3, pp. 720-724, 2014.

[14] M.E. Legaz, L. Martín, M.M. Pedrosa, C. Vicente, R. de Armas, M. Martínez, I. Medina, and C.W. Rodríguez, “Purification and partial characterization of a fructanase which hydrolyzes natural polysaccharides from sugar cane juice,” Plant Physiology, vol. 92, pp. 679-683, 1990.

[15] R. de Armas, M. Martínez, C.W. Rodriguez, M.E. Legaz, J.L. Mateos, S.V. Caffaro, and C. Vicente, “The chemical nature of high molecular mass heterofructans from cane juice,” International Sugar Journal, vol. 94, pp. 147-149, 1992.

[16] M.E. Legaz, R. de Armas, I. Medina, S.V. Caffaro, M. Martínez, J.L. Mateos, C.W. Rodríguez, and C. Vicente, “An approach to the chemical structure of sugar cane mid-molecular weight heterofructans,” Plant Physiology (Life Science Advances), vol. 11, pp. 131-140. 1992.

[17] M. Martínez, M.E. Legaz, M. Paneque, R. Domech, R. de Armas, I. Medina, C.W. Rodríguez, and Vicente, “Glycosidase activities and polysaccharide accumulation in sugar cane stalks during post-collection impairment,” Plant Science, vol, 72, pp. 193-198, 1990.

[18] B. Fontaniella, A.M. Millanes, D. Pi?ón, C.W. Rodríguez, C. Vicente, and M.E. Legaz, “Effect of leaf scald on the content of sucrose and polysaccharides of two sugar cane cultivars,” Food Science and Biotechnology, vol. 12, pp. 346–350, 2003.

[19] C. Vicente, B. Fontaniella, and M.E. Legaz, “Fructan-like polysaccharides produced by sugar beet during deterioration,” International Sugar Journal, vol. 102, pp. 250–256, 2000.

[20] M. Blanch, M.E. Legaz, and Vicente, C. “Structure and biosynthesis of a xanthan-like polysaccharide produced by Xanthomonas albilineans,” Functional Plant Science and Biotechnology, vol. 6, pp. 85-90, 2012.

[21] M. Blanch, D. Pi?on, C. Vicente, and M.E. Legaz, “Sugar cane glycoproteins are required to the production of an active UDP-glucose dehydrogenase by Xanthomonas albilineans,” Annals of Microbiology, vol. 57, pp. 217-221, 2007.

[22] R. de Armas, J.L. Mateos. S.V. Caffaro, M.E. Legaz, and C. Vicente, “Changes in sucrose crystal shape induced by cane juice fructans,” International Sugar Journal, vol. 94, pp. 141-143, 1992.

[23] G. Mantovani, I.G.Gill, and Fagioli, “Einfluss der Kristallstruktur von Nichtzuckerstoffen auf die ?nderung des Habitus von Saccharose,” Zucker, vol. 20, pp. 663-668, 1967.

[24] I.A. Khaddour, L.S.M. Bento, A.M.A. Ferreira, and F.A.N. Rocha, “Kinetics and thermodynamics of sucrose crystallization from pure solution at different initial supersaturations,” Surface Science, vol. 604, pp. 1208–1214, 2010.

[25] M. Mathlouthia, and J. Genotelleb, “Role of water in sucrose crystallization,” Carbohydrate Polymers, vol. 37, pp. 335–342, 1998.

[26] N. Faria, M.N. Pons, S. Feyo de Azevedo, F.A. Rocha, and H. Vivier, “Quantification of the morphology of sucrose crystals by image analysis,” Powder Technology, vol. 133, pp. 54–67, 2003.

[27] M.A. Godshall, M.A. Clarke, C.D. Dooley, and E.J. Roberts, “Large colorants and polysaccharide molecules in raw cane sugars,” Proceedings of the Sugar Industry Technologists, vol. 46, pp. 193-211, 1987.

[28] E.C. Vignes, “Notes on cane starch and its determination,” Proceedings of the International Society of Sugar Cane Technologists, vol. 15, pp. 1288-1295, 1974.

[29] J.P. Murray, “Filtering quality of raw sugar: influence of starch and insoluble suspended matter,” Proceedings of the Annual Congress of the South African Sugar Technologists Association, vol. 46, pp. 116-132, 1972.

[30] M. Saska, and Y. Oubrahim, “Crystallisation rate of sucrose at high impurity concentrations,” International Sugar Journal, vol. 91, pp. 109-115, 1989.

[31] M.J. Davis, A. Graves-Gillaspie, A. K. Vidaver, and R.W. Harris, “Clavibacter: a new genus containing some phytopathogenic coryneform bacteria, including Clavibacter xyli subsp. xyli sp. nov., subsp. nov. and Clavibacter xyli subsp. cynodontis subsp. nov., pathogens that cause ratoon stunting disease of sugarcane and bermudagrass stunting disease?,” International Journal of Systematic Bacteriology, vol. 34, pp. 107-117, 1984.

[32] A.H. Purcell, and D.L. Hopkins, “Fastidious xylem-limited bacterial plant pathogens,” Annual Review of Phytopathology, vol. 34, pp. 131–151, 1996.

[33] M. Blanch, C.W. Rodriguez, M.E. Legaz, and C. Vicente, “Modifications of sucrose crystallization by xanthans produced by Xanthomonas albilineans, a sugar cane pathogen,” Sugar Technology, vol. 8, pp. 255–259, 2006.

[34] E. Sánchez-Elordi, L. Morales de los Ríos, E.M. Díaz, A. ávila, M.E. Legaz, and C. Vicente, “Defensive glycoproteins from sugarcane plants induce chemotaxis, cytoagglutination and death of smut teliospores,” Journal of Plant Pathology, vol. 98, pp. 493-501, 2016.

[35] E. Sánchez-Elordi, L. Morales de los Ríos, C. Vicente, M.E. Legaz, “Sugar cane arginase competes with the same fungal enzyme as a false quorum signal against smut teliospores,” Phytochemistry Letters. Vol. 14, pp. 115-122, 2015.

[36] M.C. Molina, and C. Vicente, “Correlationships between enzymatic activity of lectins, putrescine content and chloroplast damage in Xanthoria parietina phycobionts,” Cell Adhesion and Communication, vol. 3, pp. 1-12, 1995.

[37] M.E. Legaz, M.M. Pedrosa, R. de Armas, C.W. Rodríguez, V. de los Ríos, and C. Vicente, “Separation of soluble glycoproteins from sugar cane juice by capillary electrophoresis,” Analytica Chimica Acta, vol. 372, pp. 201-208, 1998.

[38] X. Wu, S.R. Kalidindi, C. Necker, and A. Salem, “Prediction of crystallographic texture evolution and anisotropic stress–strain curves during large plastic strains in high purity a-titanium using a Taylor-type crystal plasticity model,” Acta Materialia, vol. 55, pp. 423–432, 2007.

[39] G. Mantovani, G. Vaccari, G. Sgualdino, D. Aquilano, and M. Rubbo, “Coloring matter inclusions in sucrose crystals,” Proceedings of the IX Congress of International Society of Sugar Cane Technologists, pp. 663-669, 1986.