Neuromedicine

Download PDF (204.5 KB) PP. 13 - 18 Pub. Date: November 3, 2018

DOI:

• Nato Bukia^*
  Ivane Beritashvili Center of Experimental Biomedicine, Tbilisi, Georgia
• Giorgi Kekelia
  Ivane Beritashvili Center of Experimental Biomedicine, Tbilisi, Georgia
• Marina Butskhrikidze
  Ivane Beritashvili Center of Experimental Biomedicine, Tbilisi, Georgia
• Lamara Machavariani
  Ivane Beritashvili Center of Experimental Biomedicine, Tbilisi, Georgia
• Militsa Svanidze
  Ivane Beritashvili Center of Experimental Biomedicine, Tbilisi, Georgia

In recent years, electro-magnetic field (EMF) exposure is tested as a tool for diagnosis and treatment of the wide range of neurological and psychological disorders (including epilepsy). In the present study, we examined the effects of acoustic range EMF on emotional-motivated behavior in genetically epilepsy-prone rats (GEPRs) of Krushinsky-Molodkina strain in Open field test. Methods. For this reason, generator of EMF with original coil design was used. The optimal parameters of EMF, which partially or fully inhibited the behavioral manifestation of seizure were established during pilot experiments. These were 10-15 kHz, 1-1,5 milliTesla, duration 20 min. Before and after EMF exposure in open field test, parameters of emotional-motivated behavior - entering the center, numbers of crossed squares, head raise, vertical stands, the frequency and duration of grooming, number of fecal boluses and urination were registered; Data reliability was assessed by using parametric and non-parametric techniques, with the use of one- and two-way layout of factorial analysis. Results and Discussion. On the background of a 5-days exposure of electric-magnetic field (EMF) the number of crossed squares (from 100±8 to 120±12, p≤0.01), hole reflex (from 17±2 to 24±3, p≤0.01) and head lifts (from 11 ±2 to 14±4, p≤0.05) were increased. The number of vertical stands (16±2 to 12±1 P≤ 0.05), duration (from 21 ±2 to 15±4, p≤0.05) and the number of grooming episodes (from 75 ±7 to 45±6 P≤0.01), as well as the number of fecal boluses and the frequency of urination were decreased. These findings suggest that acoustic range magnetic field decreases anxiety degree and consequently, enhances locomotor and exploratory activity of the GEPRs. A 5-days exposure EMF to inbred white rats did not change significantly locomotion/exploratory activity in open field test. Conclusion. Acoustic range EMF can be applied for suppression of behavioral manifestation of seizure. These data support a potential role of EMF exposure in changing emotion regulation of anxiety, but the mechanisms of such influence are not known. Proposed biological mechanisms in anti-seizure and anxiolytic effects of EMF exposure in GEPRs might include normalization of neuroendocrine, neurotransmitter, and/or neurotrophic factors.

Electromagnetic field; seizure; rats; general behavior

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