Isaac Scientific Publishing

Environmental Pollution and Protection

Production and Characterization of Melanin from Streptomyces Cavourensis Strain RD8 Using Response Surface Optimization

Download PDF (632.1 KB) PP. 168 - 178 Pub. Date: December 1, 2017

DOI: 10.22606/epp.2017.24002

Author(s)

  • Riddhi Naresh Dholakiya
    Marine Biotechnology and Ecology Division, CSIR-Central Salt & Marine Chemicals Research Institute, Bhavnagar (India).
  • Madhava Anil Kumar
    Marine Biotechnology and Ecology Division, CSIR-Central Salt & Marine Chemicals Research Institute, Bhavnagar (India).
  • Kalpana H. Mody*
    Marine Biotechnology and Ecology Division, CSIR-Central Salt & Marine Chemicals Research Institute, Bhavnagar (India).

Abstract

Streptomyces cavourensis strain RD8, a marine actinomycete capable to produce melanin with high antioxidant activity was screened and optimized the production using the chemometric statistical tools. The results from the optimization study revealed that the melanin production could be enhanced when medium is supplemented with L-tyrosine, 0.15, casein, 1.0 and sodium nitrate, 1.0 (g/100 mL) at pH 7.0 and 30°C at 120 rpm. The purified melanin exhibited potential antibacterial and antioxidant activities. The stability of melanin with respect to antioxidant activity against pH, temperature and salts were also observed. The outcome of the study suggested that S. cavourensis strain RD8 would be a potential candidate for melanin production with minimum medium components which could furthermore nurture as natural antioxidants in the food, cosmetic and pharmaceutical industries.

Keywords

Chemometric; melanin; optimization; Streptomyces cavourensis

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